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HLA Testing & Typing

Nutritional Health | Genetic Testing Services | Kashi Clinical Laboratories

Kashi Clinical Laboratories provides full-service HLA testing with the leading HLA typing technology, rapid turnaround times, competitive pricing, and the utmost attention to quality.

We partner with:

  • Leading national and international transplant and transfusion centers
  • Cord blood centers
  • Be The Match (NMDP)
  • International Registries
  • Contract research organizations
  • Biopharma Clinical Trials/CDX
  • Academic investigators

Indications

HLA-typing is inevitably needed for many types of research on T cell immunology, all flow cytometric work on HLA multimers, and most functional cellular assays such as ELISpot. Correlating the immune response of the subjects with their HLA type is a critical step in success of carrying out clinical trials for vaccine and vaccine development efforts.

HLA alleles are associated with several diseases and they are implicated in many types of immune response. Determination of a subject’s HLA type may reveal critical information in ascertaining drug efficacy or outcome of a disease investigation. Some HLA alleles are known to modulate the immune response of a patient to certain therapeutic products that may lead to heightened drug susceptibility. HLA typing of subjects is the key to establishing preventative measures to such responses and pursuing other treatment options.

Level 1: Intermediate Resolution HLA Testing by SSOP

Intermediate resolution HLA typing results include a subset of alleles sharing the digits in the first field of their allele name. This resolves major allele groups to 4 digits, with some degeneracy such as: A*02:01/02:02/02:05/02:36.

PCR-SSOP

Testing by sequence-specific oligonucleotide probes (SSOP)

Intermediate resolution HLA typing results include a subset of alleles sharing the digits in the first field of their allele name. This resolves major allele groups to 4 digits, with some degeneracy such as: A*02:01/02:02/02:05/02:36.

Level 2: High Resolution HLA Testing by SBT and NGS

High resolution HLA typing resolves alleles to a specific 4-digit allele, with occasional degeneracy that results in a set of alleles specifying and encoding the same protein sequence for the peptide binding region of an HLA molecule.

NGS

Next Generation Sequencing (NGS)

Next Generation Sequencing (NGS) provides an ultra-high resolution HLA typing for HLA-A, -B, -C, -DR, -DQ and -DP locus alleles. Additionally, NGS offers the capability of multiplexing the samples which can significantly reduce the turnaround time when HLA typing a large number of samples.

PCR-SSOP

Testing by sequence-specific oligonucleotide probes (SSOP)

Intermediate resolution HLA typing results include a subset of alleles sharing the digits in the first field of their allele name. This resolves major allele groups to 4 digits, with some degeneracy such as: A*02:01/02:02/02:05/02:36.

PCR-SBT

Sequence Based Typing (SBT)

Sequence Based Typing (SBT) directly identifies DNA sequence from target HLA genes. DNA amplification is performed using locus specific primers accompanied by cycling amplification buffers, Taq DNA polymerase and sample genomic DNA. The resulting product is treated and cycle sequenced. The final reactions are purified, denatured and loaded on an automated sequencing instrument for detection. The nucleotide chronology and resulting HLA subtype is determined by multicolor, fluorescence-based sequencing.

Antibody Testing | HLA Antibody Testing | Kashi Clinical Laboratories

HLA Typing

Indications

The human major histocompatibility complex (MHC), or human leukocyte antigen (HLA) complex, comprises several genetic loci on the short arm of chromosome 6. Seven of these loci encode two distinct classes of highly polymorphic cell surface antigens. Class I MHC antigens include HLA-A, HLA-B, and HLA-C, each encoded by a separate locus. Class II MHC molecules include HLA-DR, HLA-DQ, and HLA-DP each having varying degrees of alloimmunity.

Many alleles exist for each locus of the Class I and Class II of HLA genes. An extensive sequence polymorphism is present in the second exon of Class II loci and in the second and third exons of Class I loci. These polymorphic regions encode the peptide-binding groove of the MHC molecule and are important to disease susceptibility and transplantation. KCL uses the Sequence-Based Typing (SBT) methods, including Next Generation Sequencing (NGS) and Sanger Sequencing, to determine HLA-A, B, C and HLA-DR, DQ, DP high-resolution type for haematopoietic stem cell patients and their selected unrelated donors. In addition, HLA alleles have been implicated in susceptibility or resistance to a variety of autoimmune, infectious, and oncologic disorders. HLA association studies must use typing techniques with high resolution that distinguish the HLA subtypes.

Significant associations have been found between the HLA alleles and certain disorders or deficiencies in many disciplines, including rheumatology, nephrology, neurology, endocrinology, gastroenterology, respiratory, ophthalmology, infectious disease, dermatology, immunology and psychology. SBT helps define the possible associated HLA subtypes and is of interest for R & D projects concerned with T cell mediated immune response. Determination of the individual HLA marker is often used diagnostically in immunotherapy and immunomodulation approaches.

Method

Sequence Based Typing (SBT) provides a higher resolution HLA typing for HLA-A, -B, -C, -DR, -DQ and -DP locus alleles. The typing obtained by this technology is often used for typing the recipients and donors involved in stem cell transplants.  In addition, this technique provides a comprehensive typing for pharmacologic and disease association studies. Next Generation Sequencing (NGS) provides an ultra-high resolution HLA typing for HLA-A, -B, -C, -DR, -DQ and -DP locus alleles. Additionally, NGS offers the capability of multiplexing the samples which can significantly reduce the turnaround time when HLA typing a large number of samples.  The typing obtained by NGS technology is often used for typing the recipients and donors involved in stem cell transplants as well as for pharmacologic and disease association studies.

PCR-SSO

Reverse SSO hybridization is used to determine HLA-A, -B, -C, -DR, -DQ and -DP locus types at an intermediate level of resolution. These tests are used for screening potential bone marrow donors. If matched, individuals typed at this level of resolution may later require higher resolution testing.

PCR-SBT

Sequence Based Typing (SBT) provides a higher resolution HLA typing for HLA-A, -B, -C, -DR, -DQ and -DP locus alleles. The typing obtained by this technology is often used for typing the recipients and donors involved in stem cell transplants. In addition, this technique provides a comprehensive typing for pharmacologic and disease association studies.

NGS

Next Generation Sequencing (NGS) provides an ultra-high resolution HLA typing for HLA-A, -B, -C, -DR, -DQ and -DP locus alleles. Additionally, NGS offers the capability of multiplexing the samples which can significantly reduce the turnaround time when HLA typing a large number of samples. The typing obtained by NGS technology is often used for typing the recipients and donors involved in stem cell transplants as well as for pharmacologic and disease association studies.

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Kashi Laboratories works directly with providers to make ordering tests as easy as possible.

To request environmental testing at Kashi Labs, please visit the Order Now page and contact us to get started.

Kashi Laboratories works directly with providers to make ordering tests as easy as possible.

To request toxicology testing at Kashi Labs, please visit the Order Now page and contact us to get started.

What can we help you find?

Place a one-time order for genetic testing at order.kashilab.com.

If you are an existing provider, please sign in to place an order through the online portal. If you are new to Kashi and would like to create an account, please contact us.

Kashi Laboratories works directly with providers to make ordering tests as easy as possible.

Place a one-time order by contacting us through the form at the link below, or get started by logging in to your online portal.

Ordering a kit is easy! To purchase a Private Testing Kit, please visit the link below and follow the steps provided.

BoneMarrowTest.com is a service of Kashi Clinical Laboratories. For more information regarding Private HLA Testing or to order a test kit, please visit BoneMarrowTest.com.

*By clicking the link above you will be redirected to BoneMarrowTest.com.

Antibody Testing | HLA Antibody Testing | Kashi Clinical Laboratories

Kashi Labs has partnered with ThermoFisher to bring MMDx to our patients and providers.

Find more information regarding MMDx for Molecular Biopsy Assessment, sample reports, and free specimen collection kit orders.

*By clicking the link above you will be redirected to the ThermoFisher website.