Kashi Clinical Laboratories supports your SNP (single-nucleotide polymorphism) experiments using QuantStudio Real-Time PCR instrumentation. This platform not only detects the amount of fluorescence through each cycle of polymerase chain reactions, it confidently distinguishes between more ambiguous genotypes. It also provides the option of real-time data, allowing for higher sensitivity with lower DNA input, more accurate analysis of results, and faster productivity.

Kashi uses both inventoried and custom Taqman assays from Applied Biosystems to perform SNP genotyping. Taqman 5′-nuclease assays are mixtures of allele-specific primers and minor groove binding probes that identify and amplify polymorphisms, generating a fluorescent signal indicating the presence of either, or both, targeted alleles.

Potential applications of SNP genotyping include providing insight into the molecular basis of disease, pharmacogenetics, and the ability to link genetic variations with phenotypic changes.

Performance specifications include:

  • Both 96-well and 384-well plate platforms
  • Simple workflow
  • Low reaction volumes require little input DNA
  • Shorter run times allow for higher throughput of samples