Kashi Clinical Laboratories provides full service HLA Testing with the leading HLA typing technology, rapid turnaround times, competitive pricing, and the utmost attention to quality. We serve research scientists, academic investigators and Contract Research Organizations (CRO).

Indications

HLA-typing is inevitably needed for many types of research on T cell immunology, all flow cytometric work on HLA multimers, and most functional cellular assays such as ELISpot. Correlating the immune response of the subjects with their HLA type is a critical step in success of carrying out clinical trials for vaccine and vaccine development efforts.

HLA alleles are associated with several diseases and they are implicated in many types of immune response. Determination of a subject’s HLA type may reveal critical information in ascertaining drug efficacy or outcome of a disease investigation. Some HLA alleles are known to modulate the immune response of a patient to certain therapeutic products that may lead to heightened drug susceptibility. HLA typing of subjects is the key to establishing preventative measures to such responses and pursuing other treatment options.

Testing

Two levels of HLA Testing services to fit your needs:

  • Level 1: Intermediate Resolution HLA Testing by SSOP
  • Level 2: High Resolution HLA Testing by sequencing (SBT) or Next Generation Sequencing (NGS)

Level 1: Intermediate Resolution HLA Testing by SSOP

Intermediate resolution HLA typing results include a subset of alleles sharing the digits in the first field of their allele name. This resolves major allele groups to 4 digits, with some degeneracy such as: A*02:01/02:02/02:05/02:36.

PCR-SSOP PCR-SSOP hybridization is used to determine HLA typing at an intermediate level of resolution. The methodology combines locus-specific amplification of genomic DNA by PCR and hybridization with sequence-specific oligonucleotide probes (SSOP). The probes are designed to match nucleotide sequences at polymorphic sites of exons 2 and 3 for HLA alleles. The genotypes for these loci are assigned by their unique hybridization patterns.

Level 2: High Resolution HLA Testing by SBT and NGS

High resolution HLA typing resolves alleles to a specific 4-digit allele, with occasional degeneracy that results in a set of alleles specifying and encoding the same protein sequence for the peptide binding region of an HLA molecule.

NGS  Next Generation Sequencing (NGS) provides an ultra-high resolution HLA typing for HLA-A, -B, -C, -DR, -DQ and -DP locus alleles. Additionally, NGS offers the capability of multiplexing the samples which can significantly reduce the turnaround time when HLA typing a large number of samples.
PCR-SBT Sequence Based Typing (SBT) directly identifies DNA sequence from target HLA genes. DNA amplification is performed using locus specific primers accompanied by cycling amplification buffers, Taq DNA polymerase and sample genomic DNA. The resulting product is treated and cycle sequenced. The final reactions are purified, denatured and loaded on an automated sequencing instrument for detection. The nucleotide chronology and resulting HLA subtype is determined by multicolor, fluorescence-based sequencing.

Turnaround Time

Standard Service: 7 days

Next Day Service: 24 hours – Samples analyzed the same day they arrive and report sent the next day.

HLA Loci Routinely Tested

Our HLA core laboratory performs thousands of HLA typing each year. Within the MHC region, the laboratory routinely types classical Class I and Class II loci as presented in table below:

Class I Class II
HLA-A HLA-DRB1; DRB3/4/5
HLA-B HLA-DQA1
HLA-C HLA-DQB1
MICA;MICB HLA-DPB1

Sample Types and Order Handling:

Kashi Clinical Laboratories offers testing on various sample types that have been validated at the laboratory. These include fresh or frozen blood, genomic DNA, buccal swabs, cryopreserved cells and tissues.

  • There is NO price difference in the type of specimen sent
  • There is no special handling or special processing charge for various listed sample types